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MIC3018: Recombinant DNA Technology (Elective: 3 credits)

Learning Outcomes
1. Perform experiments and procedures of recombinant DNA technology.
2. Apply molecular concepts of recombinant DNA technology.

Course Synopsis
This course allows students to gain skills in recombinant DNA techniques for various
applications in biomedical science. It includes the techniques of isolating target genes,
preparation of competent cells, gene cloning, transformation, SDS-PAGE, protein
expression, western blotting, protein quantitation and chip-based analysis. Students
will also learn the strategy and consideration for standard gene cloning and cloning
expression experiments.

Reference Texts
th
1. Brown, T. A. (2015) Gene cloning and DNA analysis (7 edition). Blackwell
Publishing.
2. Leland J.C., Ara K., Peter B. K., Margaret V.W. (2016) Handbook of Molecular and
Cellular Methods in Biology and Medicine (3 edition). Taylor & Francis.
rd

Course Coordinator
Dr. Kee Boon Pin
bpkee@um.edu.my
03-79676601

Course Assessment
Course will be assessed by Continuous Assessment (100%)

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Biomedical Science Programme Guidebook 2024/2025