Page 53 - Handbook PG 20182019

Faculty of Science Postgraduate Booklet, Session 2018/2019

Medium of Instruction:
English

Transferable Skills:
Data interpretation

Humanity Skill:
CS2, CPTS2

References:
1. D.A Skoog, F.J. Holler and S.R. Crouch,”Principles of Instrumental Analysis” 6th Ed 2007,
Thomson Brooks/Cole Publ, CA, USA
2. R. Kellner, J.M. Mermet, M. Otto, V. Valcarcel and H.M. Widmer, Analytical Chemistry: A
Modern Approach to Analytical Science, 2nd Ed. 2005, John Wiley & Sons, NY
3. H. W. Williard, L. L. Meritt,Jr., J. A. Dean & F. A. Settle,Jr., Instrumental Methods of Analysis,
7th Edition, 1988, Wadworth, Ic.
4. S. Petrozzi, “Practical Instrumental Analysis: Methods, Quality Assurance and Laboratory
Management” 2012, John Wiley & Sons, NY.

SQC7004 Chromatographic Analysis

Gas chromatography (GC) is one of the most widely used analytical techniques. GC is used to
separate complex mixtures of different molecules based on their physical properties, such as polarity
and boiling point. It is an ideal tool to analyze gas and liquid samples containing many hundreds or
even thousands of different molecules, allowing the analyst to identify both the types of molecular
species present and their concentrations. The course content includes: general principle of GC,
sample injection techniques, column and column selection, theory of separation, detectors,
qualitative and quantitative analysis using GC and some basic applications of GC.

High-performance liquid chromatography (HPLC) is a chromatographic technique used to separate a
mixture of which is non-volatile. HPLC play an important and critical role in the field such as
pharmaceutical and food industries. The importance of HPLC uses in pharmaceutical industries falls
under the stringent regulations established by the U.S. Food and Drug Administration (FDA). This
requires all pharmaceutical companies to detect the quality of their products by using the HPLC
before allowing them to sell it in the global market. The course content includes: basic theory of
liquid chromatography, sample injection, major components of HPLC, mode of HPLC, mobile phase,
column and column selection, theory of separation, detectors, basic troubleshooting, qualitative and
quantitative analysis using HPLC.

Assessment Methods:
Continuous Assessment: 50%
Final Examination: 50%

Medium of Instruction:
English

Transferable Skills:
GC and HPLC data interpretation

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