Page 208 - handbook 20152016

Faculty of Science Handbook, Session 2015/2016

SIL 2009 RECOMBINANT DNA TECHNOLOGY SIL 2011 GENETIC RESOURCES AND
CONSERVATION
Introduction to DNA recombinant technology; basic gene
cloning and gene analysis; vector; enzymes; methods of This course covers the following topics: Biological diversity
DNA restriction and DNA ligation; methods to introduce (ecosystem, species, genetics), resources and ownership
foreign DNA into host cells; screening of clones; clones of the Malaysian forest, estimating and characterizing
characterisation; enzymatic manipulation of DNA and genetic diversity, inbreeding depression, population
RNA; radioactive and non-radioactive probe labelling; fragmentation, genetic management in the wild and
Southern & Northern hybridisations, colony, plaque and captivity, use of biotechnology & genetic tools in
dot blotting; radioactive and non-radioactive conservation, forensics and DNA barcoding, population
hybridisations; plasmid, lambda & M13 phages and viability analysis.
cosmid-vector cloning systems, PCR; site directed
mutagenesis and protein engineering; DNA sequencing; Assessment Methods:
and gene mapping. Next genetation sequencing and Continuous Assessment: 40%
technologies. Practical. Final Examination: 60%

Assessment Methods: Medium of Instruction:
Continuous Assessment: 40% English
Final Examination: 60%
Soft Skills
Medium of Instruction: CS3, CT2
English
Main References:
Soft Skills 1. Frankham, R., Ballou, J.D., Briscoe, D.A. (2010)
CS3, CT7, LL2, KK4, EM3 Introduction to Conservation Genetics. Cambridge
University Press.
Main References: 2. Allendorf, F.W., Luikart, G., Aitken, S.N. (2013)
1. Brown TA 2001, Gene Cloning, March 2010, ©2010,
Wiley-Blackwell Conservation and the Genetics of Populations. Wiley-
2. Primrose SB, RM Twyman & RW Old 2014, Principles Blackwell
of Gene Manipulation, 8th ed. Blackwell Sc. 3. Primack, R.B (2006). Essentials of Conservation
3. Sambrook J. & DW Russell 2001, Molecular Cloning, a Biology. Sinauer Massachusetts, USA
laboratory manual, 3rd ed.CSH Lab Press.

SIL 2010 IMMUNOGENETICS SIL 2012 PLANT GENETICS AND GENE
MANIPULATION
The course gives an understanding on the importance of
the immune system in ensuring immunity. The introduction The course introduces principles of genetics and selection
is a discussion on the cells involved in the innate and techniques used in plant breeding. Techniques used in
adaptive systems. An in-depth explanation on the clonal biotechnology for gene manipulation will also be
selection theory, rearrangement process of the discussed. Case studies and application of molecular-
receptors,T cell thymic education, B cell selection and based technology and practical sessions will be
production of antibodies/immunoglobulins will be given. conducted.
The role of the major histocompatibilty complex in
processing and presentation of antigens, activation and Assessment Methods:
signal transduction of T and B cells and importance of Continuous Assessment: 40%
cytokines regulation in immune resposes will be Final Examination: 60%
discussed. Important immune and effector mechanisms in
inflammation, phagocytosis and complement will be given Medium of Instruction:
the main focus. The last section of the course will give an English
overview of how each component signals the downstream
components in a cascade to generate an immune Soft Skills
response with the cytokines as regulators, and helper T CS4, CT2
cells in their central function with the complement system
as the main effector component. Main References:
1. Slater A, Scott NW and Fowler MR (2003) Plant
Assessment Methods: Biotechnology: The Genetic Manipulation of Plants
Continuous Assessment: 40% 2. Molecular Markers in Plants (2012) Robert J. Henry.
Final Examination: 60% John Wiley & Sons
3. Principles of Plant Genetics and Breeding (2012)
Medium of Instruction: George Acquaah John Wiley & Sons
English

Soft Skills SIL 2013 ANIMAL GENETICS AND GENE
CS3, CT3, TS3, LL2 MANIPULATION

Main References: The course is an introduction to the genetic
1. Immunology 5th edition Richard A. Goldsby, Thomas J. principles,variousbreeding techniques and manipulation of
Kindt, Barbara, A. Osborne and Janis Kuby genes for genetic improvement of animal.
2. Cellular and Molecular Immunology 7th edition Abul K.
Abbas, Andrew, H. Lichtman & Shiv Pillai. Assessment Methods:
3. Immunobiology: The Immune System in Health and Continuous Assessment: 40%
Disease 4th edition, Charles A. Janeway, Paul Travers, Final Examination: 60%
Mark Walport and J Donald Capra.

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