Page 138 - Bachelor of Biomedical Sciences

MIC3018: Recombinant DNA Technology (Elective: 3 credit hours)

Learning Outcomes
1. Perform experiments and procedures of recombinant DNA technology.
2. Apply molecular concepts of recombinant DNA technology.

Course Synopsis

This course allows students to gain skills in recombinant DNA techniques for various applications in
biomedical science. It includes the techniques of isolating target genes, preparation of competent
cells, gene cloning, transformation, SDS-PAGE, protein expression, western blotting, protein
quantitation and chip-based analysis. Students will also learn the strategy and consideration for
standard gene cloning and cloning expression experiments.

Reference Texts

1. Brown, T. A. (2015) Gene cloning and DNA analysis (7 edition). Blackwell Publishing.
th
2. Leland J.C., Ara K., Peter B. K., Margaret V.W. (2016) Handbook of Molecular and Cellular
Methods in Biology and Medicine (3 edition). Taylor & Francis.
rd

Course Coordinator

Dr. Kee Boon Pin

bpkee@um.edu.my

03-79676601

Course Assessment

Course will be assessed by Continuous Assessment (100%)

Page | 137 Biomedical Science Programme Guidebook 2023/24