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Faculty of Science Handbook, Session 2017/2018

5.L. R. Stephen, Fundamental principles of polymeric 2D-NMR: COSY, HETCOR/HMQC/HSQC, and HMBC for
materials, John Wiley, 1993. establishing molecular connectivity; 1-D NOE and 2-D
NOESY for determination of stereochemistry.
SIC2006 MOLECULAR SPECTROSCOPY Mass Spectrometry
EIMS – molecular ions, isotope peaks, and fragment ions;
Basic Spectroscopy. Vibrational, rotational, electronic HREIMS and determination of molecular formula (dual-
and nuclear magnetic resonance (NMR) spectroscopy. sector, double-focussing instruments); principal
fragmentation patterns in major classes of organic
Interaction of matters withd radiation. Transition between compounds, McLafferty rearrangement and retro-Diels-
quantum states; factors affecting intensity and width of Alder processes; Modern MS methods, ionization
spectral lines. Experimental setup. Rotational, vibrational techniques: e.g., CI-, APCI-, FD-, FAB-, DART, ESI-, and
and rotational-vibrational spectroscopy. Introduction of MALDI-MS; analyzers: Quadrupole, Ion Trap, TOF;
Raman effect. Electronic spectroscopy of atoms and Hyphenated techniques: GC- and LC-MS: Common
molecules; electronic term symbols for atoms and instrument configurations: e.g., GC-EIMS, GC-TOF-, LC-
molecules. Basics of diatomic and polyatomic molecular ESI-TOF-, LC-ESI-Q-TOF-, LC-MALDITOF-, and LC-
electronic spectroscopy. MALDITOF-TOF-MS.
Magnetic nuclei in an external magnetic field. FT NMR
experiments. Boltzman distribution and population of Application of combined techniques in organic structure
energy levels. Relaxation processes and width of spectral determination.
lines. High resolution NMR spectra: chemical shift and
spin-spin coupling. First-order and second-order spectrum. Assessment Methods:
Exchange processes. Double resonance experiments. Continuous assessment: 30%
NMR spectra of solids. Final examination: 70%
Assessment Methods: Medium of instruction:
Continuous assessment: 30% English
Final examination: 70%
Soft skills:
Medium of instruction: CT1-3
English
References:
Soft skills: 1. R. M. Silverstein, F. X. Webster, D. J. Kiemle,
CT1-3 Spectroscopic identification of organic compounds, 7 th
ed., John Wiley, 2005.
References: 2. Kamaliah Mahmood dan Noorsaadah Abd Rahman,
1. C. N. Banwell, Fundamentals of Molecular Kaedah Kimia dalam pengenalpastian Sebatian
Spectroscopy, McGraw Hill, 1994. Organik, Penerbit University of Malaya, 1998.
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2. P. W. Atkins, J. D. Paula, Physical Chemistry, 9 ed., 3. P. Crews, J. Rodriguez, M. Jaspars, Organic structure
Oxford University Press, 2010. analysis, 2 nd ed., Oxford University Press, New York,
3. P. J. Hore, Nuclear Magnetic Resonance, Oxford 2009.
University Press, 1995. 4. D. Williams, I. Flemming, Spectroscopic methods in
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4. M. Diem, Modern Vibrational Spectroscopy, John Wiley, organic chemistry, 6 ed., McGraw-Hill, 2007.
1993. 5. D. L. Pavia, G. M. Lampman, G. S. Kritz, J. A. Vyvyan,
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5. R. J. Silbey, R. A. Alberty, M. G. Bawendi, Physical Introduction to spectroscopy, 4 ed., Cengage, 2009.
Chemistry, 4 ed., John Wiley & Sons, Inc, 2005.
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SIC2008 BIOMOLECULES
SIC2007 SPECTROSCOPIC METHODS IN ORGANIC
CHEMISTRY Carbohydrates: mono-, oligo-, polysaccharides – structure,
classification, nomenclature, reactions and analysis.
IR Spectroscopy
Characteristic group absorptions in organic molecules. Lipids and terpenes: fatty acids, acyl glycerols,
prostaglandins, terpenoids, steroids – structure, reactions,
UV Spectroscopy and biological relevance.
Electronic transitions and common chromophores in
organic compounds; Beer-Lambert Law and Woodward- Proteins: amino acids, peptides, and proteins – structure,
Fieser Rules. synthesis and degradation analysis.
NMR Spectroscopy Nucleic Acids: DNA, RNA and protein biosynthesis.
Brief theory and origin of the NMR experiment; CW- and
modern pulsed FT-NMR; 1 H-NMR: the chemical shift: Assessment Methods:
inductive and anisotropic effects; Spin-spin coupling: Continuous assessment: 30%
geminal and vicinal coupling, Karplus equation; examples Final examination: 70%
of H- H splitting patterns; allylic and long-range coupling;
1
1
techniques for improving the NMR spectrum: use of shift Medium of instruction:
reagents; homonuclear-decoupling; effect of higher field English
strengths, etc.; Chirality and NMR: chiral resolving agents;
13 C-NMR: natural abundance of 13 C and use of pulsed FT Soft skills:
techniques; the 13 C NMR spectrum: completely-coupled, CT1-3
completely-decoupled; off-resonance-decoupled; APT and
DEPT spectra. Carbon chemical shifts and functional References:
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groups. 1.P. Y. Bruice, Organic Chemistry, 5 ed., Pearson, 2007.
2.H. S. Stoker, General, Organic and Biological
Chemistry. 5 ed., Brooks/Cole, 2010.
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