Page 155 - handbook 20162017

Faculty of Science Handbook, Session 2016/2017

Soft Skills Medium of Instruction:
CT1, CT2, CT3, LL1, LL2 English
Main References: Soft Skills
1. Snustad, D.P. & Simmons, M.J. (2009). Principles of CS3, CT3, TS3, LL2
Genetics. 5th ed. John Wiley.
2. Stansfield, W., Colome, J.S. & Cano, R.J. (1996). Main References:
Schaum’s Outlines: Molecular and Cell Biology. 1. Immunology 5th edition Richard A. Goldsby, Thomas J.
McGraw-Hill. Kindt, Barbara, A. Osborne and Janis Kuby
3. Weaver R.F. (2011). Molecular Biology. 5th ed. 2. Cellular and Molecular Immunology 7th edition Abul K.
McGraw-Hill. Abbas, Andrew, H. Lichtman & Shiv Pillai.
3. Immunobiology: The Immune System in Health and
Disease 4th edition, Charles A. Janeway, Paul Travers,
SIL 2009 RECOMBINANT DNA TECHNOLOGY Mark Walport and J Donald Capra.

Introduction to DNA recombinant technology; basic gene
cloning and gene analysis; vector; enzymes; methods of SIL 2011 GENETIC RESOURCES AND
DNA restriction and DNA ligation; methods to introduce CONSERVATION
foreign DNA into host cells; screening of clones; clones
characterisation; enzymatic manipulation of DNA and This course covers the following topics: Biological diversity
RNA; radioactive and non-radioactive probe labelling; (ecosystem, species, genetics), resources and ownership
Southern & Northern hybridisations, colony, plaque and of the Malaysian forest, estimating and characterising
dot blotting; radioactive and non-radioactive genetic diversity, inbreeding depression, population
hybridisations; plasmid, lambda & M13 phages and fragmentation, genetic management in the wild and
cosmid-vector cloning systems, PCR; site directed captivity, use of biotechnology & genetic tools in
mutagenesis and protein engineering; DNA sequencing; conservation, forensics and DNA barcoding, population
and gene mapping. Next genetation sequencing and viability analysis.
technologies. Practical.
Assessment Methods:
Assessment Methods: Continuous Assessment: 40%
Continuous Assessment: 40% Final Examination: 60%
Final Examination: 60%
Medium of Instruction:
Medium of Instruction: English
English
Soft Skills
Soft Skills CS3, CT2
CS3, CT7, LL2, KK4, EM3
Main References:
Main References: 1. Frankham, R., Ballou, J.D., Briscoe, D.A. (2010)
1. Brown TA 2001, Gene Cloning, March 2010, ©2010, Introduction to Conservation Genetics. Cambridge
Wiley-Blackwell
2. Primrose SB, RM Twyman & RW Old 2014, Principles University Press.
of Gene Manipulation, 8th ed. Blackwell Sc. 2. Allendorf, F.W., Luikart, G., Aitken, S.N. (2013)
3. Sambrook J. & DW Russell 2001, Molecular Cloning, a Conservation and the Genetics of Populations. Wiley-
laboratory manual, 3rd ed.CSH Lab Press. Blackwell
3. Primack, R.B (2006). Essentials of Conservation
Biology. Sinauer Massachusetts, USA
SIL 2010 IMMUNOGENETICS
The course gives an understanding on the importance of SIL 2012 PLANT GENETICS AND GENE
the immune system in ensuring immunity. The introduction MANIPULATION
is a discussion on the cells involved in the innate and
adaptive systems. An in-depth explanation on the clonal
selection theory, rearrangement process of the The course introduces principles of genetics and selection
receptors,T cell thymic education, B cell selection and techniques used in plant breeding. Techniques used in
biotechnology for gene manipulation will also be
production of antibodies/immunoglobulins will be given.
The role of the major histocompatibilty complex in discussed. Case studies and application of molecular-
processing and presentation of antigens, activation and based technology and practical sessions will be
signal transduction of T and B cells and importance of conducted.
cytokines regulation in immune responses will be Assessment Methods:
discussed. Important immune and effector mechanisms in Continuous Assessment: 40%
inflammation, phagocytosis and complement will be given
the main focus. The last section of the course will give an Final Examination: 60%
overview of how each component signals the downstream Medium of Instruction:
components in a cascade to generate an immune English
response with the cytokines as regulators, and helper T
cells in their central function with the complement system
as the main effector component. Soft Skills
CS4, CT2
Assessment Methods: Main References:
Continuous Assessment: 40%
Final Examination: 60% 1. Slater A, Scott NW and Fowler MR (2003) Plant
Biotechnology: The Genetic Manipulation of Plants

150