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Faculty of Science Handbook, Session 2017/2018

Jones & Bartlett Publishers. SIL 2010 IMMUNOGENETICS
3. Stufflebeam, C.E. (1989). Genetics of Domestic Animals.
Prentice Hall. The course gives an understanding on the importance of
the immune system in ensuring immunity. The introduction
is a discussion on the cells involved in the innate and
SIL 2008 MOLECULAR GENETICS adaptive systems. An in-depth explanation on the clonal
selection theory, rearrangement process of the receptors,T
This course includes topics involved in eukaryotic DNA cell thymic education, B cell selection and production of
replication,gene transcription and translation. The topics antibodies/immunoglobulins will be given. The role of the
covered: Eukaryotic genome, C-value paradox, histones major histocompatibilty complex in processing and
and non-histone proteins, heterochromatin and presentation of antigens, activation and signal transduction
euchromatin, classes of DNA, DNA replication in of T and B cells and importance of cytokines regulation in
eukaryotes, transcription, RNA polymerases I, II and III, immune responses will be discussed. Important immune
gene regulation in eukaryotes, various control and and effector mechanisms in inflammation, phagocytosis
regulatory elements, pre-mRNA processing and alternative and complement will be given the main focus. The last
splicing, RNA processing events (rRNA, tRNA, trans- section of the course will give an overview of how each
splicing, RNA-editing), posttranscriptional control of gene component signals the downstream components in a
expression, transcriptional regulation (DNA binding cascade to generate an immune response with the
domains), and transcriptional regulation through signal cytokines as regulators, and helper T cells in their central
transduction pathway. function with the complement system as the main effector
component.
Assessment Methods:
Continuous Assessment: 40% Assessment Methods:
Final Examination: 60% Continuous Assessment: 40%
Final Examination: 60%
Medium of Instruction:
English Medium of Instruction:
English
Soft Skills
CS3, CT2, LL2 Soft Skills
CS3, CT2, TS3, LL2
Main References:
1. Snustad, D.P. & Simmons, M.J. (2009). Principles of Main References:
Genetics. 6th ed. John Wiley. 1. Immunology 5th edition Richard A. Goldsby, Thomas J.
2. Genetic Analysis and Principles (Brooker). Kindt, Barbara, A. Osborne and Janis Kuby
3. Other Basic Genetics text books. 2. Cellular and Molecular Immunology 7th edition Abul K.
Abbas, Andrew, H. Lichtman & Shiv Pillai.
3. Immunobiology: The Immune System in Health and
SIL 2009 RECOMBINANT DNA TECHNOLOGY Disease 4th edition, Charles A. Janeway, Paul Travers,
Mark Walport and J Donald Capra.
Introduction to DNA recombinant technology; basic gene
cloning and gene analysis; vector; enzymes; methods of
DNA restriction and DNA ligation; methods to introduce SIL 2011 GENETIC RESOURCES AND
foreign DNA into host cells; screening of clones; clones CONSERVATION
characterisation; enzymatic manipulation of DNA and RNA;
radioactive and non-radioactive probe labelling; Southern & This course covers the following topics: Biological diversity
Northern hybridisations, colony, plaque and dot blotting; (ecosystem, species, genetics), resources and ownership
radioactive and non-radioactive hybridisations; plasmid, of the Malaysian forest, estimating and characterising
lambda & M13 phages and cosmid-vector cloning systems, genetic diversity, inbreeding depression, population
PCR; site directed mutagenesis and protein engineering; fragmentation, genetic management in the wild and
DNA sequencing; and gene mapping. Next generation captivity, use of biotechnology & genetic tools in
sequencing and technologies. Practical. conservation, forensics and DNA barcoding, population
viability analysis.
Assessment Methods:
Continuous Assessment: 40% Assessment Methods:
Final Examination: 60% Continuous Assessment: 40%
Final Examination: 60%
Medium of Instruction:
English Medium of Instruction:
English
Soft Skills
CS3, CT2, LL2 Soft Skills
CS3, CT2
Main References:
1. Brown TA 2010, Gene Cloning, March 2010, ©2010, Main References:
Wiley-Blackwell 1. Frankham, R., Ballou, J.D., Briscoe, D.A. (2010)
2. Primrose SB, RM Twyman & RW Old 2014, Principles Introduction to Conservation Genetics. Cambridge
of Gene Manipulation, 8th ed. Blackwell Sc.
3. Sambrook J. & DW Russell 2001, Molecular Cloning, a University Press.
laboratory manual, 3rd ed.CSH Lab Press. 2. Allendorf, F.W., Luikart, G., Aitken, S.N. (2013)
Conservation and the Genetics of Populations. Wiley-
Blackwell
3. Primack, R.B (2006). Essentials of Conservation Biology.
Sinauer Massachusetts, USA

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