Page 174 - buku panduan 20192020

Faculty of Science Handbook, Session 2019/2020

SIC2006 MOLECULAR SPECTROSCOPY Mass Spectrometry
EIMS – molecular ions, isotope peaks, and fragment ions;
Basic Spectroscopy. Vibrational, rotational, electronic HREIMS and determination of molecular formula (dual-
and nuclear magnetic resonance (NMR) spectroscopy. sector, double-focussing instruments); principal
fragmentation patterns in major classes of organic
Interaction of matters withd radiation. Transition between compounds, McLafferty rearrangement and retro-Diels-
quantum states; factors affecting intensity and width of Alder processes; Modern MS methods, ionization
spectral lines. Experimental setup. Rotational, vibrational techniques: e.g., CI-, APCI-, FD-, FAB-, DART, ESI-, and
and rotational-vibrational spectroscopy. Introduction of MALDI-MS; analyzers: Quadrupole, Ion Trap, TOF;
Raman effect. Electronic spectroscopy of atoms and Hyphenated techniques: GC- and LC-MS: Common
molecules; electronic term symbols for atoms and instrument configurations: e.g., GC-EIMS, GC-TOF-, LC-
molecules. Basics of diatomic and polyatomic molecular ESI-TOF-, LC-ESI-Q-TOF-, LC-MALDITOF-, and LC-
electronic spectroscopy. MALDITOF-TOF-MS.
Magnetic nuclei in an external magnetic field. FT NMR
experiments. Boltzman distribution and population of Application of combined techniques in organic structure
energy levels. Relaxation processes and width of spectral determination.
lines. High resolution NMR spectra: chemical shift and
spin-spin coupling. First-order and second-order spectrum. Assessment Methods:
Exchange processes. Double resonance experiments. Continuous assessment: 30%
NMR spectra of solids. Final examination: 70%

Assessment Methods: Medium of instruction:
Continuous assessment: 30% English
Final examination: 70%
Soft skills:
Medium of instruction: CT1-3
English
References:
Soft skills: 1. R. M. Silverstein, F. X. Webster, D. J. Kiemle,
CT1-3 Spectroscopic identification of organic compounds, 7 th
ed., John Wiley, 2005.
References: 2. Kamaliah Mahmood dan Noorsaadah Abd Rahman,
1. C. N. Banwell, Fundamentals of Molecular Kaedah Kimia dalam pengenalpastian Sebatian
Spectroscopy, McGraw Hill, 1994. Organik, Penerbit University of Malaya, 1998.
th
2. P. W. Atkins, J. D. Paula, Physical Chemistry, 11 ed., 3. P. Crews, J. Rodriguez, M. Jaspars, Organic structure
nd
Oxford University Press, 2018. analysis, 2 ed., Oxford University Press, New York,
3. P. J. Hore, Nuclear Magnetic Resonance, Oxford 2009.
University Press, 1995. 4. D. Williams, I. Flemming, Spectroscopic methods in
th
4. M. Diem, Modern Vibrational Spectroscopy, John Wiley, organic chemistry, 6 ed., McGraw-Hill, 2007.
1993. 5. D. L. Pavia, G. M. Lampman, G. S. Kritz, J. A. Vyvyan,
th
5. R. J. Silbey, R. A. Alberty, M. G. Bawendi, Physical Introduction to spectroscopy, 4 ed., Cengage, 2009.
th
Chemistry, 4 ed., John Wiley & Sons, Inc, 2005.
SIC2008 BIOMOLECULES

SIC2007 SPECTROSCOPIC METHODS IN ORGANIC Carbohydrates: mono-, oligo-, polysaccharides – structure,
CHEMISTRY classification, nomenclature, reactions and analysis.

IR Spectroscopy Lipids and terpenes: fatty acids, acyl glycerols,
Characteristic group absorptions in organic molecules. prostaglandins, terpenoids, steroids – structure, reactions,
and biological relevance.
UV Spectroscopy
Electronic transitions and common chromophores in Proteins: amino acids, peptides, and proteins – structure,
organic compounds; Beer-Lambert Law and Woodward- synthesis and degradation analysis.
Fieser Rules.
Nucleic Acids: DNA, RNA and protein biosynthesis.
NMR Spectroscopy
Brief theory and origin of the NMR experiment; CW- and Assessment Methods:
modern pulsed FT-NMR; H-NMR: the chemical shift: Continuous assessment: 30%
1
inductive and anisotropic effects; Spin-spin coupling: Final examination: 70%
geminal and vicinal coupling, Karplus equation; examples
1
1
of H- H splitting patterns; allylic and long-range coupling; Medium of instruction:
techniques for improving the NMR spectrum: use of shift English
reagents; homonuclear-decoupling; effect of higher field
strengths, etc.; Chirality and NMR: chiral resolving agents; Soft skills:
13
13 C-NMR: natural abundance of C and use of pulsed FT CT1-3
13
techniques; the C NMR spectrum: completely-coupled,
completely-decoupled; off-resonance-decoupled; APT and References:
th
DEPT spectra. Carbon chemical shifts and functional 1. P. Y. Bruice, Organic Chemistry, 5 ed., Pearson, 2007.
groups. 2. H. S. Stoker, General, Organic and Biological
th
Chemistry. 5 ed., Brooks/Cole, 2010.
2D-NMR: COSY, HETCOR/HMQC/HSQC, and HMBC for 3. T. K. Lindhorst, Essentials of Carbohydrate Chemistry
rd
establishing molecular connectivity; 1-D NOE and 2-D and Biochemistry, 3 ed., Wiley, 2006.
NOESY for determination of stereochemistry. 4. F.D. Gunstone, J. L. Harwood, A. J. Dijkstra, The Lipid
rd
Handbook, 3 ed., CRC Press, 2007.

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